| 背景品系 | 小鼠名稱 | 表達(dá)位置 | 基因編輯方式 | 應(yīng)用說(shuō)明 |
| C57BL/6 | Alb-cre | 肝臟 | Tg | cre expressed in hepatocytes; very efficient in achieving liver-specific recombination when crossed with liver specific floxed alleles. |
| C57BL/6 | CAG-CreERT | 胚胎，廣泛表達(dá) | Tg | tamoxifen-inducible cre; widespread pattern of expression; tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice |
| C57BL/6 | CaMK2-Cre | 腦組織 | Tg | This transgene directs the expression of Cre recombinase under the control of the alpha subunit of the calcium/calmodulin-dependent protein kinase II promoter. Cre recombinase activity was detected in the CA1 region of the hippocampus. |
| C57BL/6 | Carp-cre | 腦組織 | Tg | 腦組織特異Cre轉(zhuǎn)基因小鼠 |
| C57BL/6 | Cd19-cre | B細(xì)胞 | Tg | In frame insertion of a cre recombinase gene into the first coding exon followed by an frt-flanked neomycin cassette. A frameshift mutation was also introduced into exon 5. This allele expresses cre recombinase specifically in B lineage cells throughout development. |
| C57BL/6 | CMV-CRE | 廣泛表達(dá) | Tg | This transgene expresses Cre recombinase under the control of a human cytomegalovirus (CMV) promoter, active in most cells and tissues. |
| C57BL/6 | Col1-cre | 成骨細(xì)胞 | Tg | Inducible cre recombinase activity is detected in the osteoblasts of the long bones of the limbs and in the ribs, vertebrae and calvaria in 18.5 embryonic day aged and 18 day old mice. |
| C57BL/6 | Col2-gfp | 軟骨細(xì)胞 | Tg | An EGFP fluorescent reporter is expressed from the murine Col2a1 promoter, providing an in vivo/in vitro tool for the studies of chondrocyte differentiation and cartilage matrix synthesis. |
| C57BL/6 | Col2-cre | 軟骨細(xì)胞 | Tg | cre expression is directed to cells of the chondrogenic lineage (cartilage) during embryogenesis and postnatally |
| C57BL/6 | Fabp4-cre | 白色和棕色脂肪組織 | Tg | The FABP4-Cre transgene has a mouse Fabp4 promoter directing expression of Cre recombinase. These FABP4-Cre transgenic mice are a Cre-lox tool useful for deletion of floxed sequences in brown and white adipose tissue. |
| C57BL/6 | GFAP-Cre | 腦、脊髓中星膠質(zhì)細(xì)胞 | Tg | |
| C57BL/6 | GFPA-Cre | Tg | ||
| C57BL/6 | Gpr126-Cre | Tg | ||
| C57BL/6 | Gαq Lysom-Cre | Tg | ||
| C57BL/6 | Ipf1-cre | 這些突變的突變小鼠具有loxP位點(diǎn)，位于Pdx1基因的外顯子2A的側(cè)面 | Tg | These floxed mutant mice possess loxP sites flanking exon 2A of the Pdx1 gene. This strain may be useful for generating conditional mutations in applications related to the pancreatic development, pancreatic beta cell function, tumorigenesis, tumor progression and metastasis. |
| C57BL/6 | Lck-cre | T淋巴細(xì)胞表達(dá) | Tg | This transgenic strain expresses Cre under the control of the Lck (lymphocyte protein tyrosine kinase) promoter, enabling thymocyte-specific excision of loxP-flanked sequences of interest. The Lck gene is primarily expressed by T lymphocytes and it plays a key role in the selection and maturation of developing T-cells in the thymus and in the activity of mature T-cells. |
| C57BL/6 | Lgr5-CreERT2 | Tg | ||
| C57BL/6 | Lgr5-EGFP-Ires-Cre(ERT2) | 表達(dá)EGFP和CreERT2融合蛋白 | Tg | Heterozygous mice harbor an Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function and expresses EGFP and CreERT2 fusion protein. When these mice mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Lgr5-expressing cells of the offspring. These mice may be useful for lineage-tracing or marking Lgr5-expressing stem cells of the small intestine. |
| B6.129P2 | Lyz2-Cre | re介導(dǎo)的對(duì)切除的區(qū)域的切除會(huì)導(dǎo)致敲除等位基因 | Tg | Exon 4 of the mouse Itgav gene is flanked by loxP sites in these αvflox mice. Cre-mediated excision of the floxed region results in a knock-out allele useful in studies of ulcerative colitis, autoimmunity, vascular development, and cancer. |
| C57BL/6 | Lyz2-Cre | Tg | ||
| C57BL/6 | MHC-Cre | 心臟特異性α肌球蛋白重鏈（Myh6）啟動(dòng)子驅(qū)動(dòng)cre | Tg | The cardiac-specific alpha myosin-heavy chain (Myh6) promoter drives expression of cre in this transgenic strain. The promoter induces greater than 90% recombination in cardiac muscle cells. |
| FVB | MMTV-Cre | 含有Cre重組酶的轉(zhuǎn)基因 | Tg | These MMTV-Cre/Line D mice express a transgene containing Cre recombinase with widespread expression (but not in oocytes) under control the MMTV LTR promoter. |
| C57BL/6 | Mx1-cre | 能夠在與該轉(zhuǎn)基因雜交的亞麻突變體的子代發(fā)育過(guò)程中的任何時(shí)候進(jìn)行“敲除” | Tg | The Cre recombinase is under the control of the Mx1 promoter, which can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA (such as poly I:C); enabling the "knockout" at any time during development in progeny of a floxed mutant mated to this transgenic. |
| B6.FVB | Myh11-cre/ERT2 | 平滑肌細(xì)胞中基因的條件缺失 | Tg | These mice may be useful for the conditional deletion of genes in smooth muscle cells. |
| C57BL/6 | Nestin-Cre | 巢蛋白啟動(dòng)子驅(qū)動(dòng)的Cre重組酶在中樞神經(jīng)系統(tǒng)和周圍神經(jīng)系統(tǒng) | Tg | This strain is commonly known as nestin-Cre. Nestin promotor driven Cre recombinase is expressed in the central and peripheral nervous system, including neuronal and glial cell precursors. There are reports of Cre recombinase activity in a few isolated kidney and heart cells. Hemizygous nestin-cre mice exhibit mild hypopituitarism, growth retardation, impaired fear response, and a metabolic phenotype. Mice also show a strong impairment in the acquisition of both contextual- and cued-fear conditioning. |
| C57BL/6 | Nestin-creERT | 他莫昔芬誘導(dǎo)的Cre重組酶 | Tg | Nestin-Cre/ERT2 transgenic mice express tamoxifen-inducible Cre recombinase in adult and developing mouse brain, including neuronal and glial cell precursors. |
| C57BL/6 | Nestin-gfp | 針對(duì)胚胎腦神經(jīng)上皮的增強(qiáng)型綠色熒光蛋白 | Tg | Nestin-GFP mice express an enhanced green fluorescent protein (EGFP) directed to the neuroepithelium of the embryonic brain using the regulatory elements of the Nes (nestin) gene. This mutant mouse strain may be useful in visualizing neural stem and progenitor cells in embryonic and adult brains. |
| C57BL/6 | Optogenetic-Cre | Tg | ||
| C57BL/6 | Osx-Cre | 造血干細(xì)胞的維持 | Tg | Exon 2 of the mouse Cxcl12 (chemokine (C-X-C motif) ligand 12) gene is flanked by loxP sites in this conditional targeted mutation strain. Cre recombinase-mediated excision of the floxed region enables tissue/cell-specific knockouts of the gene, useful in studies of hematopoietic stem cell maintenance or other CXCL12-dependent processes. |
| C57BL/6 | Pdx1-Cre | 胰腺特異性條件突變 | Tg | These Pdx-Cre transgenic mice express Cre recombinase under the control of the mouse Pdx1 promoter and may be useful for generating pancreas-specific conditional mutations. |
| C57BL/6 | Pdx1-cre/Esr1 | 條件性胰腺細(xì)胞敲除 | Tg | This tamoxifen-inducible Pdx1-Cre/Esr1 strain may be used to create conditional pancreatic cell knockouts when crossed with strains carrying floxed alleles. |
| C57BL/6 | PP2ac-Cre | Tg | ||
| FVB | Rank-Cre | 代謝紊亂以及膽汁酸，脂質(zhì)和葡萄糖代謝的調(diào)節(jié) | Tg | Fxrfl (loxP::exon::loxP::Δneo) is a floxed allele - loxP sites flank the last exon of the Nr1h4 gene. Removal of the floxed sequence creates a null allele. These mice may be useful in studying metabolic disorders, as well as the regulation of bile acid, lipid and glucose metabolism. Δneo) |
| C57BL/6 | Rosa26-lacZ | KI | ||
| C57BL/6 | Rosa26-Lsl-EGFP | 綠色熒光 | KI | |
| C57BL/6 | Rosa26-tdTomato | 紅色熒光 | KI | |
| C57BL/6 | RUNX2-CRE | 成骨研究 | KI | These Runx2-BioFL knock-in mice express a RUNX2 protein with a C terminus biotin/FLAG tag. They are suitable for use in ChIP-seq applications and in studies of osteogenesis. |
| C57BL/6 | SPC-Cre | 腦、骨腫瘤，淋巴瘤 | Tg | Trp53 knockout mutant mice develop tumors at three to six months of age. They are suitable for use in applications related to the study of familial breast cancers such as Li-Fraumeni syndrome as well as research of lung, brain and bone tumors, lymphoma and leukemia, and other rare cancers. |
| C57BL/6 | UCP1-egfp | 該突變小鼠品系在肥胖，代謝穩(wěn)態(tài)和適應(yīng)性生熱研究中有用 | KI | These Ucp knock-out mice are sensitive to cold temperatures, exhibit altered metabolism and are resistant to diet induced obesity when maintained at 20°C. This mutant mouse strain may be useful in studies of obesity, metabolic homeostasis, and adaptive thermogenesis. |
| C57BL/6 | Vav-cre | 造血細(xì)胞條件性突變 | Tg | Vav-iCre transgenic mice have the mouse HS21/45 control regions directing expression of an optimized variant of Cre recombinase (iCre) to hematopoietic cells (and their progenitors), and may be useful for generating conditional mutations in hematopoietic cells. |
| C57BL/6 | Villin-Cre | 小腸和大腸的絨毛和隱窩上皮細(xì)胞中表達(dá)Cre重組酶 | Tg | These Vil1-cre mice express Cre recombinase in villus and crypt epithelial cells of the small and large intestines and may be useful in studies of intestinal organogenesis. In contrast to Villin-cre transgenic mice from founder line 1000 (Stock No. 021504) that are reported be absent of Cre recombinase activity in gonads, these Villin-cre transgenic mice from founder line 997 are reported to have a very low level (<1%) of cells with Cre recombinase activity in the testes. |
| C57BL/6 | Villin-CreERT2 | 腸上隱窩絨毛軸 | Tg | vil-Cre-ERT2 mice carry a transgene with tamoxifen inducible Cre recombinase under the control of the 9 kb mouse Vil1, villin 1, promoter, and may be useful for generating specific targeted mutants for studies of the crypt-villus axis of the intestinal epithelium. |
| C57BL/6 | α-MHC-Cre | 心肌細(xì)胞 | Tg | The cardiac-specific alpha myosin-heavy chain (Myh6) promoter drives expression of cre in this transgenic strain. The promoter induces greater than 90% recombination in cardiac muscle cells. |
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